Multi-Q: Fully automated tool for multiplexed protein quantitation data analysis.
Mass spectrometry has emerged as one of the most powerful high throughput techniques for protein analysis. Furthermore, the iTRAQ labeling techniques, which can analyze up to four sets of samples at a time, is one of the most up-to-date methods for multiplexed protein identification and quantitation in this field. Such quantitation is based on multidimentional chromatography in conjunction with mass spectrometry and tandem mass spectrometry. Multi-Q is such a fully automated tool to expedite the analysis of vast amounts of spectral data.
Multi-Q is designed for iTRAQ-based protein quantitation. It can analyze up to four data sets at the same time. The input data are in mzXML and pepXML format. mzXML data format is converted from raw data files while pepXML is converted from the result of protein identification. Multi-Q uses only uniuque peptides for protein quantificaiton. It uses the processes of determing peptide ratio, normalization, and protein ratio to obtained protein ratio results.

Demo site URL: http://ms.iis.sinica.edu.tw/Multi-Q