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Journal of Information Science and Engineering, Vol. 27 No. 2, pp. 749-760 (March 2011)

Automatic Counting Cancer Cell Colonies using Fuzzy Inference System*

SHENG-FUU LIN, HSIEN-TSE CHEN AND YI-HSIEN LIN+
Department of Electrical Engineering
National Chiao Tung University
Hsinchu, 300 Taiwan
E-mail: sflin@mail.nctu.edu.tw
+Institute of Traditional Medicine
National Yang-Ming University
Taipei, 112 Taiwan
+Division of Radiotherapy
Cheng Hsin Rehabilitation Medical Center
Taipei, 112 Taiwan

This paper examines the efficacy of liver cancer treatment using HA22T cancer cells specific to the Taiwanese population. The Clonogenic Assay is the current standard method for detecting liver cancer. This paper uses image processing technology and a fuzzy inference system to identify in-vitro colonies of cancer cells. A scanner was first used to capture an image of the culture dish. This image was then analyzed using image processing techniques and the Hough transform to establish the relative position of the dish. Image segmentation was accomplished by image differencing, while feature extraction was based on the features specific to the image. Decision-making was then carried out using a fuzzy inference system to calculate the number of colonies within the image. In summary, this paper proposes a fuzzy cancer cell colony identification system based on a fuzzy inference system (FIS) that successfully identifies cancer cell colonies that are indiscernible to the naked eye.

Keywords: clonogenic assays, cancer, feature extraction, image processing, fuzzy inference system (FIS)

Full Text () Retrieve PDF document (201103_21.pdf)

Received June 11, 2009; revised September 24 & December 3, 2009 & March 3, 2010; accepted April 26, 2010.
Communicated by Suh-Yin Lee.
* This work was supported partially by the National Science Council of Taiwan, R.O.C., under Grant No. NSC 96-2221-E-009-238.