TIGP -- Profiling genome wide DNA methylation in Tuber melanosporum (truffle)
- LecturerProf. Pao-Yang Chen (Institute of Plant and Microbial Biology, Academia Sinica)
Host: TIGP Bioinformatics Program - Time2013-03-19 (Tue.) 14:00 ~ 15:10
- LocationAuditorium 106 at new IIS Building
Abstract
Pe'rigord black truffle (Tuber melanosporum Vittad.) is a gastronomic delicacy produced in southern Europe. The 125-megabase (Mb) genome of T. melanosporum is the largest sequenced fungal genome to date. In this project, we performed whole bisulfite sequencing on the fruit body and the free-living mycelium, and observed a mosaic pattern of DNA methylation across the genome. The methylation is mainly in transposons but absent in genes, indicating that the fungal DNA methylation system appears to be very different from plant and
mammalian ones. While the transposon methylation shows anti-correlation with its expression, we also found that transposons are less methylated in the vicinity of highly expressed genes compared to poorly expressed genes. Lastly, I will introduce the procedure of generating whole genome DNA methylation profiles in my lab.