Speaker: Dr. Hsei-Wei Wang Date: Thu Oct 7, 2010 at 14:00 ~ 15:00 Place: Auditorium 106 at new IIS Building Abstract: In this post-genomic era it is clear that the sum of the cellular gene expression state determines the cellular phenotypes. The advance in genomics technology permits the recognition of disease-associated roteins or genomic traits that may serve as clinical markers or survival indexes. MicroRNAs (miRNAs) are small regulatory RNAs that derive from distinctive hairpin transcripts. How might miRNAs regulate physiological or pathological activities has become an evolving research field. The indication of the abundance of miRNA genes came from sequencing small RNAs from mammals, flies, and worms. Microarray platforms have been applied extensively in microRNA research. However, some miRNAs are expressed only in a limited number of cells or through a limited portion of development, and their rarity makes them difficult to detect by microarray or Sanger sequencing of cloned small RNA-derived cDNAs. The development of RNA-Seq technique, one of the next generation sequencing (NGS) technology applications, help to map human transcriptpme in many individuals and cell types. The challenge we now face is to keep up the bioinformatics analysis, to understand this blueprint, and to determine how errors lead to disease. Bearing these in mind, we developed bioinformatics pipelines for NSG and microRNA array data analyses and setup wetlab functional assay platforms for validating the very existence of the discovered novel miRNAs and for studying the function of mined miRNAs. Further efforts will be to apply cloud computing technologies to facilitate NGS data processing, as well as to further elucidate the function of miRNAs.