Institute of Information Science, Academia Sinica

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Seminar

TIGP (BP) - Using single-cell RNA-seq technology to reveal the role of tissue resident memory (TRM) T-cells in Breast Cancer

  • LecturerDr. Agus Salim (Department of Mathematics and Statistics, La Trobe University)
    Host: TIGP BP Program
  • Time2018-09-27 (Thu.) 14:00 – 16:00
  • LocationAuditorium 101 at IIS new Building
Abstract

  Using Chromium 10X technology, we performed single-cell RNA sequencing (scRNA-seq) of 6,311 T cells isolated from human BCs. We demonstrate that BCs with a high number of Tumor Inflitrating Lymphocytes (TILs) contained CD8+ T cells with features of tissue-resident memory T (TRM) cell differentiation and that these CD8+ TRM  cells expressed high levels of immune checkpoint molecules and effector proteins. A CD8+ TRM gene signature developed from the scRNA-seq data was significantly associated with improved patient survival in early-stage triple-negative breast cancer (TNBC) and provided better prognostication than CD8 expression alone.